1. Its What’s on the Inside That Counts: An Effective, Efficient, and Streamlined Method for Quantification of Octocoral Symbiodiniaceae and Chlorophyll
Date Submitted: 10/25/2019 06:36 PM
Average star voting: (4.77/5 stars and 23769 reviews)
Summary: Ocean warming driven bleaching is one of the greatest threats to zooxanthellate cnidarians in the Anthropocene. Bleaching is the loss of Symbiodiniaceae, chlorophyll, or both from zooxanthellate animals. To quantify bleaching and recovery, standardised methods for quantification of Symbiodiniaceae and chlorophyll concentrations have been developed for reef-building scleractinian corals, but no such standard method has been developed for octocorals. For stony corals, quantification of Symbiodiniaceae and chlorophyll concentrations often relies on normalisation to skeletal surface area or unit of biomass (i.e. protein, ash-free dry weight (AFDW)). Stiff octocorals do not change their volume, as such studies have used volume and surface area to standardise densities, but soft-bodied octocorals can alter their size using water movement within the animal; therefore, Symbiodiniaceae and chlorophyll cannot accurately be measured per unit of surface area and are instead measured in units of Symbiodiniaceae and chlorophyll per µg of host protein or AFDW. Though AFDW is more representative of the full biomass composition than host protein, AFDW is more time and resource intensive. Here we provide a streamlined methodology to quantify Symbiodiniaceae density, chlorophyll concentration, and protein content in soft-bodied octocorals. This technique uses minimal equipment, does not require freeze-drying or burning samples to obtain ash weight, and is effective for down to 0.2 grams wet tissue. Bulk samples can be centrifuged, the Symbiodiniaceae pellet washed, and the supernatant saved for protein analysis. This efficient technique allows for clean, easy to count samples of Symbiodiniaceae with minimal animal protein contamination. Chlorophyll a and c2 extractions occurs at different rates, with chlorophyll a taking 24 hours to extract completely at 4°C and chlorophyll c2 taking 48 hours. Finally, we found that where necessary, wet weight may be used as a proxy for protein content, but the correlation of protein and wet weight varies by species and protein should be used when possible. Overall, we have created a rapid and accurate method for quantification of bleaching markers in octocorals.
Match with the search results: Sep 9, 2021 … Its What’s on the Inside That Counts: An Effective, Efficient, and Streamlined Method for Quantification of Octocoral Symbiodiniaceae and …
2. It’s What’s Inside That Counts… or Is It? – Blog – The Sound
Date Submitted: 08/04/2021 08:39 AM
Average star voting: (3.91/5 stars and 98968 reviews)
Summary: People value internal beauty above external beauty. But while our beliefs, and the marketing messages we hear from beauty brands, may be focused more and more on Inner beauty, regardless of gender and age outward appearances still fuel success. Read our new Creative Analytics report for more insights….
Match with the search results: Apr 6, 2020 … People place inner beauty above outer beauty · How We View Ourselves: People rate their own internal beauty higher than their external. · How We …
3. Why We Must Stop Saying, “It’s What’s on the Inside that Counts”
Date Submitted: 12/23/2020 08:20 PM
Average star voting: (4.4/5 stars and 33832 reviews)
Summary: It’s what’s on the inside that counts. -Everyone I distinctly remember how I felt when someone told me, “It’s on the inside that counts.” Frustration! All through my teens and twenties, I wanted to care less about my weight or my skin or my hair. But, I didn’t know …
Match with the search results: Sep 19, 2020 … “Focus on what’s great about you…on the inside…and then you won’t care about that extra ten pounds.” In this one simple phrase we deliver a …